FIG. 1.
(A) yra1ΔRBD is synthetically lethal with a mutation in HPR1. Strain sl57 (yra1ΔRBD hpr1-57 〈pCH1122 YRA1 URA ADE3〉) was transformed with empty vector or YRA1 and HPR1 gene constructs (pFS2525 and pFS2576). Transformants were streaked on 5-FOA plates to select against wild-type pCH1122-YRA1 and examine rescue of the synthetic phenotype. (B) Various yra1 mutants are synthetically lethal with Δhpr1. The YRA1 shuffle (FSY1026) or YRA1 shuffle Δhpr1 (FSY1655) strains were transformed with vector or plasmids encoding wild-type or mutant Yra1p (LEU2 CEN). Transformants were streaked on 5-FOA plates and incubated at 25 or 37°C for 4 days. (C) sub2 mutants are synthetically lethal with Δhpr1. The SUB2 shuffle (DLY23) or the SUB2 shuffle Δhpr1 (FSY1664) strains were transformed with empty vector or plasmids encoding wild-type or mutant Sub2p (LEU2 CEN). Transformants were analyzed as described for panel B.