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Repression of a mexJ-lacZ transcriptional fusion by MexL. Cells of strains PAO238 (mexL wild type) or PAO318 (ΔmexL) containing a plasmid-encoded mexJ-lacZ transcriptional fusion (pJZ110) or the vector control (pTZ110) were grown to mid-log phase in LB medium supplemented with 100 μg of carbenicillin per ml. β-Galactosidase activity was measured in triplicate samples, and the t distribution was used to establish 95% confidence limits.
