TABLE 3.
Probing cell surface properties, molecular interactions, and elasticity
Organism | Method | Results | Reference(s) |
---|---|---|---|
Escherichia coli | Force-distance curves with cell- | Attractive hydrophobic interactions; | 23, 37, 40 |
coated probes | repulsive steric interactions; | ||
hydrophobic and electrostatic interactions | 40 | ||
Shewanella oneidensis | Force-distance curves with cell-coated probes | Adhesion between cells and mineral surfaces; adhesion peaks suggested to reflect unfolding of an outer membrane protein | 24 |
Burkholderia cepacia, Pseudomonas putida | Force-distance curves with silicon nitride probes | Electrostatic and steric interactions | 13 |
Phanerochaete chrysosporium | Force-distance curves with chemically functionalized probes | Hydrophobic and hydration interactions, mapping of surface hydrophobicity | 17 |
Magnetospirillum gryphiswaldense | Approach force curves | Elasticity of cell wall, estimate of turgor pressure | 2, 3 |
Streptococcus salivarius | Approach force curves | Softness of surface fibrils | 48 |
Methanospirillum hungatei | Depression technique | Elasticity of proteinaceous sheath | 50 |
Escherichia coli, | Depression technique | Elasticity of murein sacculi | 10, 51 |
Pseudomonas aeruginosa | |||
Deinococcus radiodurans | Retraction force curves | Stretching single hexagonally packed intermediate protomers, images of single-molecule defects | 34 |
Aspergillus oryzae | Retraction force curves | Stretching surface macromolecules | 47 |