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. 1977 Oct;271(2):537–547. doi: 10.1113/jphysiol.1977.sp012013

Properties of isolated adult rat muscle fibres maintained in tissue culture.

A Bekoff, W Betz
PMCID: PMC1353585  PMID: 926004

Abstract

1. Adult rat skeletal muscles were dissociated by collagenase treatment and trituration, and the isolated muscle fibres were maintained in vitro for 2-3 weeks. At various stages, the fibres were examined physiologically and morphologically. 2. The isolated fibres underwent some changes characteristic of muscle denervated in vivo. For instance, input resistance increased and extrajunctional acetylcholine (ACh) receptors appeared. In addition, the beginning stages of apparent muscle fibre fragmentation were observed. 3. In other respects, the cultured isolated fibres behaved differently than in vivo denervated fibres. Fibrillation developed only occasionally in vitro. The onset of ACh supersensitivity was slower (6 days) than after denervation in vivo (2-3 days). Some fibres developed localized regions of destriation, which apparently was due to loss of in-register alignment of myofibrils.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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