FIG. 4.
Intracellular transport of proteins is not affected in the pancreas of Rab3D-deficient mice. Pancreatic lobules derived from wild-type and KO mice were incubated for 5 min in medium containing 3H-labeled amino acids. After chase to cold medium containing either no stimulants (0 μM) or 0.5 μM carbachol, aliquots of the medium (A) and the lobules (B) were removed as indicated, and trichloroacetic acid-precipitable radioactivity was determined. The values were normalized to the DNA content of the homogenates. Values are means of two different animals; bars indicate the range.