FIG. 3.
Expression of human STAT2 in mouse cells permits STAT degradation. NIH 3T3 cells (3T3) and a derivative NIH 3T3 line expressing human STAT2 (3T3/HuS2) were infected with 10 PFU of HPIV2 (H) or SV5 (S)/cell. Lysates prepared 16 h p.i. were separated by SDS-PAGE and processed for immunoblotting along with control uninfected cells (C). (A) Immunoblot with antiserum that recognizes HPIV2 and SV5 nucleocapsid proteins (NPSV5). (B) Immunoblot with antiserum specific for murine STAT2 (MuSTAT2). (C) Immunoblot with antiserum specific for murine STAT1 (MuSTAT1) that recognizes both full-length murine STAT1α and truncated STAT1β derived from alternative mRNA splicing. (D) Immunoblot with antiserum specific for human STAT2 (HuSTAT2).