Figure 4.
Pre- and postsynaptic molecular architecture of neuromuscular junctions at P70. (A,B) Innervation at the level of individual synapses in mutant (A) and control (B) diaphragm shows no differences. Neurofilament is in green and AChR in red. (C,D) Immune staining of presynaptic synaptophysin (green) and r-bgt-stained postsynaptic AChR (red) superpose perfectly in end-plates of diaphragm muscle of AChRγ(ɛ/ɛ) (C) and control mice (D). (E,F) MuSK staining (green) in cross-sections of mutant (E) and control soleus muscle (F) counter-labelled with r-bgt (red).