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Fig. 6. Nap1p is a karyopherin specificity determinant in vitro. Binding of recombinant MBP–Kap114p (A), MBP–Kap121p (B) or MBP–Kap123p (C) (120 nM each) was tested on immobilized GST, GST–H2A1–46 and GST–H2B1–52 (1.6 µM) in the presence or absence of recombinant Nap1p (0.8 µM). The bound fractions were analyzed as described.
