FIG. 2.
A fixed number of phage with serial dilutions of host cells. (A) Approximately 400 PFU of P1 transducing phage were incubated with serial dilutions of P1C600 host cells at the cell densities indicated for 30 min and then plated on kanamycin to select for cells that had been transduced with the reporter phagemid. (B) Approximately 400 PFU of transducing phage M13K07 were incubated with serial dilutions of ER2738 host cells at the cell densities indicated for 30 min and then plated on carbenicillin and X-Gal to select for cells that had been transduced with reporter phagemid pBlue-GFPuv. The expected number of transductants for each cell density was calculated as either N(1 − e−MOIactual) (○) or N(1 − e−MOIinput) (□). ▴, observed numbers of transductants.