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Comparison of the spectra of Nova-1 KH3 free and in an RNA complex recorded at 500 MHz and 25°C. (A) 15N HSQC spectrum of the free protein. (B) 15N HSQC of a 15N leucine selectively labeled sample of free Nova-1 KH3. (C) Plot of the normalized (see Materials and Methods) values of peak intensities of the spectrum in A versus the sequence of the protein. The peaks with intensities close to 1 are those boxed in Figure 2B ▶. (D) 15N HSQC spectrum of a 1:1 Nova-1 KH3/RNA1 complex.
