Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1990 Nov;71(3):423–427.

The function of high endothelial venules in mouse lymph nodes stimulated by oxazolone.

R E Mebius 1, J Brevé 1, A M Duijvestijn 1, G Kraal 1
PMCID: PMC1384443  PMID: 2269478

Abstract

The effects of antigenic stimulation on the expansion of T-cell dependent areas in lymph nodes of mice were studied in relation to the effects on high endothelial venules (HEV) located in this area. Lymph nodes regional to areas of skin that had been treated with solutions of oxazolone were studied at several time-points after stimulation. The following measurements were made relative to lymph nodes of untreated animals: (i) the expansion of the T-cell dependent areas in combination with the increase of HEV in this area, as detected by the HEV-specific mAb MECA-325, using morphometric analysis: (ii) the influx of FITC-labelled lymphocytes from the blood into the lymph node by FACS: (iii) the capacity of HEV to bind lymphocytes using an in vitro binding assay. Morphometry showed that T-cell dependent areas increased rapidly after stimulation with oxazolone and although the mean area of MECA-325-positive HEV had also increased, this increase lagged behind the expansion of the T-cell area. Therefore the amount of HEV per T-cell area in an antigen-stimulated lymph node was smaller than in an untreated lymph node and correlated with the percentage of FITC-labelled cells that had entered it. In a lymph node from an oxazolone-treated animal this percentage was decreased to the same order of magnitude as the area of HEV per T-cell area, but the overall binding capacity of HEV was not affected by oxazolone treatment. Antigenic stimulation therefore leads to a rapid expansion of the potential sites of lymphocyte entry into a lymph node, but the efficiency of the HEV does not change.

Full text

PDF
423

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson N. D., Anderson A. O., Wyllie R. G. Microvascular changes in lymph nodes draining skin allografts. Am J Pathol. 1975 Oct;81(1):131–160. [PMC free article] [PubMed] [Google Scholar]
  2. BURSTONE M. S. Histochemical comparison of naphthol AS-phosphates for the demonstration of phosphatases. J Natl Cancer Inst. 1958 Mar;20(3):601–615. [PubMed] [Google Scholar]
  3. Butcher E. C., Scollay R. G., Weissman I. L. Lymphocyte adherence to high endothelial venules: characterization of a modified in vitro assay, and examination of the binding of syngeneic and allogeneic lymphocyte populations. J Immunol. 1979 Nov;123(5):1996–2003. [PubMed] [Google Scholar]
  4. Butcher E. C. The regulation of lymphocyte traffic. Curr Top Microbiol Immunol. 1986;128:85–122. doi: 10.1007/978-3-642-71272-2_3. [DOI] [PubMed] [Google Scholar]
  5. Butcher E. C., Weissman I. L. Direct fluorescent labeling of cells with fluorescein or rhodamine isothiocyanate. I. Technical aspects. J Immunol Methods. 1980;37(2):97–108. doi: 10.1016/0022-1759(80)90195-7. [DOI] [PubMed] [Google Scholar]
  6. Davies A. J., Carter R. L., Leuchars E., Wallis V. The morphology of immune reactions in normal, thymectomized and reconstituted mice. II. The response to oxazolone. Immunology. 1969 Jul;17(1):111–126. [PMC free article] [PubMed] [Google Scholar]
  7. Drayson M. T., Smith M. E., Ford W. L. The sequence of changes in blood flow and lymphocyte influx to stimulated rat lymph nodes. Immunology. 1981 Sep;44(1):125–133. [PMC free article] [PubMed] [Google Scholar]
  8. Duijvestijn A. M., Kerkhove M., Bargatze R. F., Butcher E. C. Lymphoid tissue- and inflammation-specific endothelial cell differentiation defined by monoclonal antibodies. J Immunol. 1987 Feb 1;138(3):713–719. [PubMed] [Google Scholar]
  9. GOWANS J. L., KNIGHT E. J. THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257–282. doi: 10.1098/rspb.1964.0001. [DOI] [PubMed] [Google Scholar]
  10. Hall J. G., Morris B. The immediate effect of antigens on the cell output of a lymph node. Br J Exp Pathol. 1965 Aug;46(4):450–454. [PMC free article] [PubMed] [Google Scholar]
  11. Harms G., van Goor H., Koudstaal J., de Ley L., Hardonk M. J. Immunohistochemical demonstration of DNA-incorporated 5-bromodeoxyuridine in frozen and plastic embedded sections. Histochemistry. 1986;85(2):139–143. doi: 10.1007/BF00491761. [DOI] [PubMed] [Google Scholar]
  12. Hay J. B., Hobbs B. B. The flow of blood to lymph nodes and its relation to lymphocyte traffic and the immune response. J Exp Med. 1977 Jan 1;145(1):31–44. doi: 10.1084/jem.145.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hay J. B., Johnston M. G., Vadas P., Chin W., Issekutz T., Movat H. Z. Relationships between changes in blood flow and lymphocyte migration induced by antigen. Monogr Allergy. 1980;16:112–125. [PubMed] [Google Scholar]
  14. Herman P. G., Utsunomiya R., Hessel S. J. Arteriovenous shunting in the lymph node before and after antigenic stimulus. Immunology. 1979 Apr;36(4):793–797. [PMC free article] [PubMed] [Google Scholar]
  15. Herman P. G., Yamamoto I., Mellins H. Z. Blood microcirculation in the lymph node during the primary immune response. J Exp Med. 1972 Oct 1;136(4):697–714. doi: 10.1084/jem.136.4.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kamperdijk E. W., Raaymakers E. M., de Leeuw J. H., Hoefsmit E. C. Lymph node macrophages and reticulum cells in the immune response. I. The primary response to paratyphoid vaccine. Cell Tissue Res. 1978 Aug 25;192(1):1–23. doi: 10.1007/BF00231019. [DOI] [PubMed] [Google Scholar]
  17. Kraal G., Twisk A. The interaction of high endothelial venules with T and B cells in peripheral lymph nodes after antigenic stimulation. Eur J Immunol. 1984 Jun;14(6):586–588. doi: 10.1002/eji.1830140617. [DOI] [PubMed] [Google Scholar]
  18. Ledbetter J. A., Herzenberg L. A. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63–90. doi: 10.1111/j.1600-065x.1979.tb00289.x. [DOI] [PubMed] [Google Scholar]
  19. Stamper H. B., Jr, Woodruff J. J. Lymphocyte homing into lymph nodes: in vitro demonstration of the selective affinity of recirculating lymphocytes for high-endothelial venules. J Exp Med. 1976 Sep 1;144(3):828–833. doi: 10.1084/jem.144.3.828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Streeter P. R., Rouse B. T., Butcher E. C. Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes. J Cell Biol. 1988 Nov;107(5):1853–1862. doi: 10.1083/jcb.107.5.1853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. van Rooijen N. The "in situ" immune response in lymph nodes: a review. Anat Rec. 1987 Aug;218(4):359–364. doi: 10.1002/ar.1092180402. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES