Table 2.
Remethylation of Backcross Progeny from a Heterozygous zmet2-m1::Mu Plant Backcrossed to the Nonmutant Mo17 Parental Linea
| Genotype | nb | Total 5-me Cytosine (%)c |
|---|---|---|
| Mo17 wild-type parent | 3 | 24.63 a |
| zmet2-m1::Mu homozygous mutant parent | 3 | 20.50 d |
| Mo17 × zmet2-m1::Mu F1 | 3 | 22.96 c |
| Homozygous normal Zmet2 BC1 progeny | 3 | 24.05 b |
| Heterozygous zmet2-m1::Mu BC1 progeny | 3 | 23.24 c |
a Mean cytosine methylation levels are given for each family type. Percentages of 5mC content [5mC/(5mC + C)] were calculated from concentrations determined from integration of peak areas and comparisons with known standards.
b
n, number of individual plants tested. Two duplicates of each sample were analyzed by HPLC.
c Letters following means indicate groupings that were significantly different (α < 0.01).