Figure 10. Measurements of PMg2+ , Rt , and PNa /PCl ratios.
(A) PMg2+. Monolayers of stable transfected MDCK-C7 cells were grown on permeable supports and mounted in Ussing chambers. Mg2+ was added to the basolateral side, and net flux was measured by means of AAS. *P < 0.05 versus control. (B) Comparison of Rt to PMg2+. Rt of the clones ranged between 941 ± 174 Ω cm2 (T233R) and 3,406 ± 303 Ω cm2 (H71D). No correlation of PMg2+ and Rt was observed (n = 7–16). (C) PNa/PCl ratios. Dilution potentials were measured with modified Ringer’s solution on the luminal or serosal side, and the data from the 2 conditions was pooled (n = 6 per group). No significant changes were observed in cells expressing WT Cldn16 or the mutants indiced.