Figure 3.
Glomerular gene expression patterns determined by ISH in E18.5 kidneys. (A, B) Overview of the renal expression of glomerulus-expressed genes by non-radioactive ISH. (A) Podocyte-expressed genes Foxc2, Sem2, Rhpn1, Cited2, Pi15, GeneX, Nphs2 and Podxl. (B) Mesangial/juxtaglomerular-expressed genes Sfrp2, Igfbp5, Akr1b7, Ren1 and Lmo7, and endothelium-expressed gene Emcn (endomucin). (C, D) Temporal expression of podocyte (C) and mesangial/juxtaglomerular (D) markers during nephron development by non-radioactive ISH. ‘Comma' and ‘S-shaped' refer to morphologically distinct early stages of nephron development, whereas ‘capillary loop' and ‘mature' refer to morphologically distinct late stages of glomerulus development. Note that only Foxc2 and Sfrp2 are expressed at the earliest stage of nephron development, whereas all other markers appear in the podocyte and mesangial/juxtaglomerular apparatus cells during S-shaped (podocyte markers) and capillary loop stages (mesangial markers).