Abstract
The in vitro reaction between sensitized lymphoid cells and target cells has been studied in an allogeneic transplantation system in mice. Mastocytoma cells of the DBA/2 donor strain were injected into C57BL mice, and the spleens of the recipients harvested 8 days later. The immune lymphoid cells thus obtained were tested for their ability to damage in vitro cultures of target mastocytoma cells. The reaction was followed by two methods: (1) microscopic counts, and (2) ability of target cells to form colonies in a semi-solid medium after various periods of contact with immune lymphoid cells. The results show: (a) that the effect on target cells is an exponential function of the number of immune lymphoid cells employed, and (b) that the reaction becomes noticeable after 3 hours and reaches completion within 12 hours when evaluated by cloning techniques, while it takes 24–48 hours to be microscopically clearly demonstrable. Problems arising from use of both methods are discussed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BRONDZ B. D. INTERACTION OF IMMUNE LYMPHOCYTES IN VITRO WITH NORMAL AND NEOPLASTIC TISSUE CELLS. Folia Biol (Praha) 1964;10:164–176. [PubMed] [Google Scholar]
- DUNN T. B., POTTER M. A transplantable mast-cell neoplasm in the mouse. J Natl Cancer Inst. 1957 Apr;18(4):587–601. [PubMed] [Google Scholar]
- FRIEDMAN H. INHIBITION OF ANTIBODY PLAQUE FORMATION BY SENSITIZED LYMPHOID CELLS: RAPID INDICATOR OF TRANSPLANTATION IMMUNITY. Science. 1964 Aug 7;145(3632):607–609. doi: 10.1126/science.145.3632.607. [DOI] [PubMed] [Google Scholar]
- KISTLER P., NITSCHMANN H. Large scale production of human plasma fractions. Eight years experience with the alcohol fractionation procedure of Nitschmann, Kistler and Lergier. Vox Sang. 1962 Jul-Aug;7:414–424. doi: 10.1111/j.1423-0410.1962.tb03274.x. [DOI] [PubMed] [Google Scholar]
- KOPROWSKI H., FERNANDES M. V. Autosensitization reaction in vitro. Contactual agglutination of sensitized lymph node cells in brain tissue culture accompanied by destruction of glial elements. J Exp Med. 1962 Oct 1;116:467–476. doi: 10.1084/jem.116.4.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROSENAU W., MOON H. D. Lysis of homologous cells by sensitized lymphocytes in tissue culture. J Natl Cancer Inst. 1961 Aug;27:471–483. [PubMed] [Google Scholar]
- SCHINDLER R. Biochemical studies of the division cycle of mammalian cells: evidence for the premitotic period. Biochem Pharmacol. 1963 Jun;12:533–538. doi: 10.1016/0006-2952(63)90128-x. [DOI] [PubMed] [Google Scholar]
- SCHINDLER R. QUANTITATIVE COLONIAL GROWTH OF MAMMALIAN CELLS IN FIBRIN CELLS. Exp Cell Res. 1964 May;34:595–598. doi: 10.1016/0014-4827(64)90245-9. [DOI] [PubMed] [Google Scholar]
- TAYLOR H. E., CULLING C. F. CYTOPATHIC EFFECT IN VITRO OF SENSITIZED HOMOLOGOUS AND HETEROLOGOUS SPLEEN CELLS ON FIBROBLASTS. Lab Invest. 1963 Sep;12:884–894. [PubMed] [Google Scholar]
- WILSON D. B. THE REACTION OF IMMUNOLOGICALLY ACTIVATED LYMPHOID CELLS AGAINST HOMOLOGOUS LYMPHOID CELLS AGAINST HOMOLOGOUS TARGET TISSUE CELLS IN VITRO. J Cell Physiol. 1963 Dec;62:273–286. doi: 10.1002/jcp.1030620307. [DOI] [PubMed] [Google Scholar]