FIG. 5.
Cbx7 chromatin association is RNA dependent. (A) RNase treatment of 3-day-differentiated ES cells strongly diminished the accumulation of Cbx7-EGFP on the Xi. The Xi is visualized by K27me3 staining (red), and the signal of Cbx7-EGFP was enhanced using α-GFP antibody (green). (B) Degradation of Cbx7 is not observed after RNase treatment (or DNase I treatment), as detected by Western blot with GFP antibody. (C) Cbx7 is depleted from 6-day-differentiated ES cell chromatin by RNase treatment (top), while WDR5 and HP1β are not affected. See histones for equal loading (bottom).