TABLE 1.
Phagocytic index numbersa for the phagocytosis assay
| Mφ sourceb | Incubation conditions (time and concn) | PI for:
|
|
|---|---|---|---|
| Wild type | MR−/− | ||
| RPc | 15 min | 40-9 | 40-5 |
| RP | 30 min | 56-7 | 59-6 |
| TPd | 30 min | 77-13 | 83-15 |
| TP | 30 min, 100 μg of glucan/ml | 0.4-0.05 | 0.3-0.05 |
| TP | 30 min, 500 μg of mannan/ml | 79-10 | 77-6 |
a
Phagocytic index (PI) numbers indicate the average numbers of CFU of intracellular C. albicans per 10 peritoneal macrophages. To calculate the phagocytic index, 10 groups of 10 macrophages were chosen from either different parts of the same coverslip or from a different coverslip.
b
Mφ, macrophage.
c
RP, resident peritoneal Mφ.
d
TP, thioglycolate-elicited Mφ.