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. 1986 Jun;58(2):245–250.

Cyclophosphamide and dimethyl dioctadecyl ammonium bromide immunopotentiate the delayed-type hypersensitivity response to inactivated enveloped viruses.

R H Smith, B Ziola
PMCID: PMC1452649  PMID: 2423444

Abstract

Immunization of BALB/c mice with measles virus inactivated with beta-propiolactone and mixed with 100 micrograms of the cationic surface-active lipid dimethyl dioctadecyl ammonium bromide (DDA) primes for a strong virus-specific delayed-type hypersensitivity (DTH) response that peaks 1 week later. Optimal immunization and challenge doses were found to be 8 and 4 micrograms/mouse, respectively, and pretreatment with 200 mg of cyclophosphamide/kg 2 days prior to immunization significantly enhanced the DTH response. When compared to Freund's complete and incomplete adjuvants, DDA was superior for induction of DTH to inactivated purified measles virus. As DDA could be administered to animals at a site different from the measles virus antigens, or 1 day previously, and still significantly enhance the DTH response, DDA is probably acting more as an immune modulator than as a simple adjuvant. The conditions for an optimal DTH response to measles virus were also shown to be applicable to other enveloped viruses, for example, a strong DTH response was similarly generated to inactivated purified influenza PR8 virus and to herpes simplex virus type I antigens present in plasma membranes isolated from infected Vero cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Battisto J. R., Beckman K., Yen-Lieberman B. Dextran augments delayed-type hypersensitivity by interrupting one limb of the suppressor cascade. J Immunol. 1985 Apr;134(4):2131–2138. [PubMed] [Google Scholar]
  2. Coon J., Hunter R. Selective induction of delayed hypersensitivity by a lipid conjugated protein antigen which is localized in thymus dependent lymphoid tissue. J Immunol. 1973 Jan;110(1):183–190. [PubMed] [Google Scholar]
  3. Cooper M. G. Delayed-type hypersensitivity in the mouse. I. Induction and elicitation by Salmonella adelaide flagellin and its derivatives. Scand J Immunol. 1972;1(2):167–178. doi: 10.1111/j.1365-3083.1972.tb00596.x. [DOI] [PubMed] [Google Scholar]
  4. Dailey M. O., Hunter R. L. The role of lipid in the induction of hapten-specific delayed hypersensitivity and contact sensitivity. J Immunol. 1974 Apr;112(4):1526–1534. [PubMed] [Google Scholar]
  5. Griffin D. E., Mullinix J., Narayan O., Johnson R. T. Age dependence of viral expression: comparative pathogenesis of two rodent-adapted strains of measles virus in mice. Infect Immun. 1974 Apr;9(4):690–695. doi: 10.1128/iai.9.4.690-695.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kraaijeveld C. A., Jansen J., Benaissa-Trouw B., Snippe H. Delayed-type hypersensitivity in mice after infection with avirulent Semliki Forest virus. Brief report. Arch Virol. 1983;78(1-2):115–118. doi: 10.1007/BF01310865. [DOI] [PubMed] [Google Scholar]
  7. Kraaijeveld C. A., Snippe H., Harmsen M., Khader Boutahar-Trouw B. Dimethyl dioctadecyl ammoniumbromide as an adjuvant for delayed type hypersensitivity and cellular immunity against Semliki Forest virus in mice. Arch Virol. 1980;65(3-4):211–217. doi: 10.1007/BF01314537. [DOI] [PubMed] [Google Scholar]
  8. Lagrange P. H., Mackaness G. B., Miller T. E. Potentiation of T-cell-mediated immunity by selective suppression of antibody formation with cyclophosphamide. J Exp Med. 1974 Jun 1;139(6):1529–1539. doi: 10.1084/jem.139.6.1529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leung K. N., Ada G. L. Production of DTH in the mouse to influenza virus: comparison with conditions for stimulation of cytotoxic T cells. Scand J Immunol. 1980;12(2):129–139. doi: 10.1111/j.1365-3083.1980.tb00049.x. [DOI] [PubMed] [Google Scholar]
  10. Neighbour P. A., Rager-Zisman B., Bloom B. R. Susceptibility of mice to acute and persistent measles infection. Infect Immun. 1978 Sep;21(3):764–770. doi: 10.1128/iai.21.3.764-770.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith R. H., Ziola B. Induction of delayed-type hypersensitivity to measles virus in mice. Cell Immunol. 1984 Nov;89(1):20–29. doi: 10.1016/0008-8749(84)90193-x. [DOI] [PubMed] [Google Scholar]
  12. Snippe H., Belder M., Willers J. M. Dimethyl diotadecyl ammonium bromide as adjuvant for delayed hypersensitivity in mice. Immunology. 1977 Dec;33(6):931–936. [PMC free article] [PubMed] [Google Scholar]
  13. Snippe H., de Reuver M. J., Beunder J. W., van der Meer J. B., van Wichen D. F., Willers J. M. Delayed-type hypersensitivity in rabbits. Comparison of the adjuvants dimethyl dioctadecyl ammonium bromide and Freund's complete adjuvant. Int Arch Allergy Appl Immunol. 1982;67(2):139–144. [PubMed] [Google Scholar]
  14. Wright K., Ramshaw I. A. A requirement for helper T cells in the induction of delayed-type hypersensitivity. J Immunol. 1983 Apr;130(4):1596–1599. [PubMed] [Google Scholar]
  15. Ziola B., Hader W. J. Adherent cells suppress measles and herpes simplex I virus-induced blastogenesis of multiple sclerosis lymphocytes. J Neuroimmunol. 1985 Feb-Mar;7(5-6):315–330. [PubMed] [Google Scholar]
  16. van Houte A. J., Snippe H., Peulen G. T., Willers J. M. Characterization of immunogenic properties of haptenated liposomal model membranes in mice. II. Induction of delayed-type hypersensitivity. Immunology. 1981 Jan;42(1):165–173. [PMC free article] [PubMed] [Google Scholar]

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