Figure 7.
Rescue of impaired angiogenic phenotype in GPx-1 KO mice by intravenous injection of WT EPCs. A, Representative LDBF images showing improved perfusion of ischemic limbs in WT and GPx-1 KO mice by IV injection of WT but not GPx-1 KO ex vivo cultured EPCs on the day of surgery. LDBF measurements were made 14 days after the induction of ischemia. B, Quantitative analysis of ischemic/nonischemic LDBF ratio in GPx-1 KO and WT mice on postoperative day 14 with or without IV injection of ex vivo cultured EPCs from WT or GPx-1 KO mice. *P<0.01 vs control (no EPCs) and GPx-1 KO– derived EPCs for the WT recipient; *P<0.01 vs control and GPx-1 KO– derived EPC for the GPx-1 KO recipient mice (n=4). C, Quantitative analysis of capillary density in ischemic adductor muscle of WT and GPx-1 KO mice on postoperative day 14. Capillary density is expressed as the number of capillaries per high-power field (hpf) (×400); #P<0.05 vs control and GPx-1 KO– derived EPCs for the WT recipient. *P<0.01 vs control and GPx-1 KO– derived EPCs for the GPx KO recipient.