FIG. 5.
Overexpression of Uqcrc1 in an N2A cell line causes increased mitochondrial respiration. (a) Real-time PCR analysis of Uqcrc1 mRNA expression relative to GAPDH. Uqcrc1 expression in control cell line was normalized to 1. (b) Representative Western blot of whole-cell lysates from control and Uqcrc1-overexpressing N2A cells. Porin serves as a loading control. Statistical analysis of duplicate experiments indicates 1.6-fold overexpression of Uqcrc1 protein. (c) Oxygen electrode data for the ADP-uncoupled respiration rates of permeabilized control and Uqcrc1-overexpressing cells in the presence of pyruvate/malate, succinate, or TMPD/ascorbate as respiratory substrates. Significance was tested using the t test. **, P < 0.01; *, P < 0.05. Error bars indicate standard deviations.