TABLE 2.
MICs of various agents for P. aeruginosa producing different combinations of EmrEPae and MexAB-OprMa
| Strain (genotype) | MIC (μg/ml)b
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| EtBr | AF | MV | AMK | GEN | KAN | NEO | TOB | NAL | PMB | |
| HN1112 (wild type) | 256 | 32 | 8 | 0.06 (2, 8) | 0.128 (0.5, 4) | 4 (64, 128) | 0.25 (16, 64) | 0.064 (0.5, 2) | 32 | 0.25 |
| HN1113 (ΔemrEPae) | 64 | 8 | 8 | 0.06 (2, 4) | 0.064 (0.5, 4) | 2 (64, 128) | 0.064 (16, 64) | 0.064 (0.5, 2) | 16 | 0.25 |
| HN1114 (ΔmexAB-oprM) | 64 | 8 | 8 | 0.03 (2, 2) | 0.016 (0.25, 0.5) | 2 (64, 128) | 0.128 (8, 16) | 0.032 (0.5, 0.5) | 4 | 0.125 |
| HN1115 (ΔmexAB-oprM ΔemrEPae) | 32 | 4 | 8 | 0.03 (1, 2) | 0.016 (0.25, 0.5) | 1 (32, 128) | 0.032 (8, 8) | 0.016 (0.25, 0.5) | 4 | 0.125 |
| K1589 (ΔmexR ΔmexB) | 32 | 16 | 8 | 0.03 (1, 2) | 0.032 (0.25, 2) | 2 (64, 128) | 0.128 (16, 32) | 0.032 (0.5, 1) | 8 | 0.25 |
| HN1116 (ΔmexR ΔmexB ΔemrEPae) | 16 | 4 | 8 | 0.03 (1, 2) | 0.016 (0.25, 2) | 1 (64, 128) | 0.064 (8, 16) | 0.016 (0.25, 1) | 4 | 0.125 |
MIC values were determined in NB, except the values in parentheses. In addition to the agents listed here, the MICs of the following agents were determined, and some results for the following agents are mentioned in the text: spectinomycin, azlocillin, carbenicillin, cloxacillin, cefsulodin, cefuroxime, ceftriaxone, norfloxacin, ciprofloxacin, enoxacin, novobiocin, chloramphenicol, erythromycin, fusidic acid, tetracycline, trimethoprim, phosphonomycin, rifampin, vancomycin, sodium dodecyl sulfate, and triclosan. Abbreviations: EtBr, ethidium bromide; AF, acriflavine; MV, methyl viologen; AMK, amikacin; GEN, gentamicin; KAN, kanamycin; NEO, neomycin; TOB, tobramycin; NAL, nalidixic acid; PMB, polymyxin B.
Values in parentheses are MIC values obtained in MHB and LB medium as follows: MHB MIC, LB medium MIC.