FIG. 2.
TC45-D182A exits the nucleus and colocalizes with IR β-subunit in response to insulin. CHO/IR cells were transfected with pCG constructs expressing wild-type TC45 or the TC45-D182A substrate-trapping mutant (TC45D). At 24 h posttransfection, cells were serum starved and either left untreated or stimulated with 100 nM insulin (INS) for 30 min. Cells were processed for (A) epifluorescence microscopy with the monoclonal anti-TCPTP antibody CF4 or (B) for confocal microscopy with the rabbit polyclonal anti-TCPTP antibody 6228 and a mouse monoclonal IR β-subunit (IRβ) antibody as described previously (48).