Table 2.
Results of PCR Analysis of 5-FC–Resistant Tobacco Calli Obtained after Induction of DSBs by Transient Expression of I-SceI with the Single-Copy Line GU.C.USB 1
a
Histochemical staining of all 10 calli demonstrated the functional restoration of the GUS gene. Blue staining was not detected for the other calli analyzed in this experiment.
b
For a number of 5-FC–resistant calli, no PCR fragment was amplified under the conditions used. The most likely explanation is that in these calli, the DSB caused a deletion that included at least part of the primer binding sites. Alternatively, insertions too large to be amplified by PCR or other major rearrangements might be responsible for the lack of a PCR fragment.