Table 3.
Compilation of DNA Gel Blot Analysis of Plants of Lines GU.C.USB 3 and 7 Regenerated from 5-FC–Resistant Tobacco Calli Obtained after Induction of DSBs by Transient Expression of I-SceI
| GUS-Specific Acc65I Fragment (kb)
|
||||
|---|---|---|---|---|
| Line | 3.7 (HR) |
4.4 (NHEJ) |
3.7 and 4.4 (HR and NHEJ) |
Othersa |
| 3 | 6 | 18 | 0 | 15 |
| 7 | 2 | 5 | 0 | 7 |
a
The DNA gel blot pattern resulted in several cases in the presence of one or more GUS-specific fragments smaller than 3.0 kb, indicating the deletion of parts of the transgene locus. In two cases, larger bands indicative of further rearrangements were detected. However, no codA-specific sequences were detected in any line tested.