Table 1.
Purification of sAC from 950 rat testis
| AC activity
|
||||
|---|---|---|---|---|
| Protein, mg | Total units, nmol/min | Specific activity, nmol/min/mg × 100 | Fold enrichment | |
| Cytosol | 51,900 | 2,400 | 4.6 | 1 |
| Preparative DE-52 | 4,015 | 3,000 | 75 | 16 |
| Gel filtration AcA54 | 1,074 | 2,100 | 200 | 43 |
| Reactive Red | 66 | 1,200 | 1,800 | 390 |
| Source Q | 8.8 | 1,100 | 12,500 | 2,700 |
| Reactive Green | 1.8 | 380 | 21,000 | 4,600 |
| Semipreparative QA, pH = 7.4 | 0.6 | 310 | 52,000 | 11,300 |
| Analytical QA, pH = 6.8 | ||||
| #18 | 0.003 | 90 | 3,000,000 | 650,000 |
| #19 | 0.010 | 92 | 920,000 | 200,000 |
Protein concentrations determined by OD280. Units refer to nmol of cAMP formed per minute. Fold enrichment represents specific activity after each step compared to the starting specific activity. See Materials and Methods for detailed description of each purification step.