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. 1984 Aug;57(2):293–300.

Malarial parasites and tumour cells are killed by the same component of tumour necrosis serum.

J Taverne, N Matthews, P Depledge, J H Playfair
PMCID: PMC1536137  PMID: 6467675

Abstract

Tumour necrosis serum (TNS), from animals primed with macrophage activating agents and challenged with endotoxin, causes necrosis of some tumours and can kill certain tumour lines in vitro and malarial parasites in vitro and in vivo. We have tested the possibility that the same factor is responsible for killing both tumour cells and malarial parasites. In competitive inhibition experiments, parasitized erythrocytes, but not normal erythrocytes, inhibited the cytotoxicity of TNS against a tumour cell line. Conversely, the tumour cells inhibited the killing of Plasmodium yoelii in vitro by TNS. When rabbit TNS was fractionated by ion exchange chromatography followed by gel filtration and the fractions at each step were pooled according to their ability to kill the tumour cells, in vitro parasite killing activity was found to correlate with tumour cell cytotoxicity, to a final sample which was purified more than 600-fold. Our results suggest that in terms of function, at least, the same component of TNS is responsible for the killing of both tumour cells and malarial parasites.

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Selected References

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  1. Carswell E. A., Old L. J., Kassel R. L., Green S., Fiore N., Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3666–3670. doi: 10.1073/pnas.72.9.3666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clark I. A., Allison A. C., Cox F. E. Protection of mice against Babesia and Plasmodium with BCG. Nature. 1976 Jan 29;259(5541):309–311. doi: 10.1038/259309a0. [DOI] [PubMed] [Google Scholar]
  3. Clark I. A., Virelizier J. L., Carswell E. A., Wood P. R. Possible importance of macrophage-derived mediators in acute malaria. Infect Immun. 1981 Jun;32(3):1058–1066. doi: 10.1128/iai.32.3.1058-1066.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cottrell B. J., Playfair J. H., de Sousa B. Plasmodium yoelii and Plasmodium vinckei: the effects of nonspecific immunostimulation on murine malaria. Exp Parasitol. 1977 Oct;43(1):45–53. doi: 10.1016/0014-4894(77)90006-6. [DOI] [PubMed] [Google Scholar]
  5. Egyed A. The significance of transferrin-bound bicarbonate in the uptake of iron by reticulocytes. Biochim Biophys Acta. 1973 May 28;304(3):805–813. doi: 10.1016/0304-4165(73)90227-4. [DOI] [PubMed] [Google Scholar]
  6. Faulk W. P., Hsi B. L., Stevens P. J. Transferrin and transferrin receptors in carcinoma of the breast. Lancet. 1980 Aug 23;2(8191):390–392. doi: 10.1016/s0140-6736(80)90440-7. [DOI] [PubMed] [Google Scholar]
  7. Haidaris C. G., Haynes J. D., Meltzer M. S., Allison A. C. Serum containing tumor necrosis factor is cytotoxic for the human malaria parasite Plasmodium falciparum. Infect Immun. 1983 Oct;42(1):385–393. doi: 10.1128/iai.42.1.385-393.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jahiel R. I., Vilcek J., Nussenzweig R. S. Exogenous interferon protects mice against Plasmodium berghei malaria. Nature. 1970 Sep 26;227(5265):1350–1351. doi: 10.1038/2271350a0. [DOI] [PubMed] [Google Scholar]
  9. Jensen J. B., Boland M. T., Allan J. S., Carlin J. M., Vande Waa J. A., Divo A. A., Akood M. A. Association between human serum-induced crisis forms in cultured Plasmodium falciparum and clinical immunity to malaria in Sudan. Infect Immun. 1983 Sep;41(3):1302–1311. doi: 10.1128/iai.41.3.1302-1311.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kull F. C., Jr, Cuatrecasas P. Preliminary characterization of the tumor cell cytotoxin in tumor necrosis serum. J Immunol. 1981 Apr;126(4):1279–1283. [PubMed] [Google Scholar]
  11. Matthews N. Effect of human monocyte killing of tumour cells of antibody raised against an extracellular monocyte cytotoxin. Immunology. 1983 Feb;48(2):321–327. [PMC free article] [PubMed] [Google Scholar]
  12. Matthews N. Production of an anti-tumour cytotoxin by human monocytes. Immunology. 1981 Sep;44(1):135–142. [PMC free article] [PubMed] [Google Scholar]
  13. Matthews N., Ryley H. C., Neale M. L. Tumour-necrosis factor from the rabbit. IV. Purification and chemical characterization. Br J Cancer. 1980 Sep;42(3):416–422. doi: 10.1038/bjc.1980.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Matthews N. Tumour-necrosis factor from the rabbit. III. Relationship to interferons. Br J Cancer. 1979 Oct;40(4):534–539. doi: 10.1038/bjc.1979.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Matthews N., Watkins J. F. Tumour-necrosis factor from the rabbit. I. Mode of action, specificity and physicochemical properties. Br J Cancer. 1978 Aug;38(2):302–309. doi: 10.1038/bjc.1978.202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mazurek C., Chalvet H., Stiffel C. L., Biozzi G. Study of the mechanism of Corynebacterium parvum anti-tumour activity. I. Protective effect on the growth of two syngeneic tumours. Int J Cancer. 1976 Apr 15;17(4):511–517. doi: 10.1002/ijc.2910170414. [DOI] [PubMed] [Google Scholar]
  17. Ostrove J. M., Gifford G. E. Stimulation of RNA synthesis in L-929 cells by rabbit tumor necrosis factor. Proc Soc Exp Biol Med. 1979 Mar;160(3):354–358. doi: 10.3181/00379727-160-40449. [DOI] [PubMed] [Google Scholar]
  18. Playfair J. H., de Souza J. B., Taverne J. Endotoxin induced 'tumour-necrosis serum' kills a subpopulation of normal lymphocytes in vitro. Clin Exp Immunol. 1982 Mar;47(3):753–755. [PMC free article] [PubMed] [Google Scholar]
  19. Pollack S. Malaria and iron. Br J Haematol. 1983 Feb;53(2):181–183. doi: 10.1111/j.1365-2141.1983.tb02009.x. [DOI] [PubMed] [Google Scholar]
  20. Sauvaget F., Fauconnier B. The protective effect of endogenous interferon in mouse malaria, as demonstrated by the use of anti-interferon globulins. Biomedicine. 1978 Oct;29(6):184–187. [PubMed] [Google Scholar]
  21. Schultz W. W., Huang K. Y., Gordon F. B. Role of interferon in experimental mouse malaria. Nature. 1968 Nov 16;220(5168):709–710. doi: 10.1038/220709a0. [DOI] [PubMed] [Google Scholar]
  22. Scott M. T. Corynebacterium parvum as an immunotherapeutic anticancer agent. Semin Oncol. 1974 Dec;1(4):367–378. [PubMed] [Google Scholar]
  23. Taverne J., Depledge P., Playfair J. H. Differential sensitivity in vivo of lethal and nonlethal malarial parasites to endotoxin-induced serum factor. Infect Immun. 1982 Sep;37(3):927–934. doi: 10.1128/iai.37.3.927-934.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Taverne J., Dockrell H. M., Playfair J. H. Endotoxin-induced serum factor kills malarial parasites in vitro. Infect Immun. 1981 Jul;33(1):83–89. doi: 10.1128/iai.33.1.83-89.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Taverne J., Dockrell H. M., Playfair J. H. Killing of the malarial parasite Plasmodium yoelii in vitro by cells of myeloid origin. Parasite Immunol. 1982 Mar;4(2):77–91. doi: 10.1111/j.1365-3024.1982.tb00421.x. [DOI] [PubMed] [Google Scholar]
  26. Tosta C. E., Sedegah M., Henderson D. C., Wedderburn N. Plasmodium yoelii and Plasmodium berghei: isolation of infected erythrocytes from blood by colloidal silica gradient centrifugation. Exp Parasitol. 1980 Aug;50(1):7–15. doi: 10.1016/0014-4894(80)90003-x. [DOI] [PubMed] [Google Scholar]
  27. Weddernburn N., Campa M., Tosta C. E., Henderson D. C. The effect of malaria on the growth of two syngeneic transplantable murine tumours. Ann Trop Med Parasitol. 1981 Dec;75(6):597–605. doi: 10.1080/00034983.1981.11687490. [DOI] [PubMed] [Google Scholar]
  28. Wozencraft A. O., Dockrell H. M., Taverne J., Targett G. A., Playfair J. H. Killing of human malaria parasites by macrophage secretory products. Infect Immun. 1984 Feb;43(2):664–669. doi: 10.1128/iai.43.2.664-669.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. van Bockxmeer F. M., Morgan E. H. Transferrin receptors during rabbit reticulocyte maturation. Biochim Biophys Acta. 1979 Apr 18;584(1):76–83. doi: 10.1016/0304-4165(79)90237-x. [DOI] [PubMed] [Google Scholar]

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