TABLE 1.
Subcellular distribution of GFP fused to the N-terminal fragments of several Zn binuclear cluster proteins in yeast kap mutant strains
| Straina | Distribution of GFP inb:
|
|||
|---|---|---|---|---|
| AlcR (1-75) | Gal4p (1-147) | Pdr3p (1-100) | PrnA (1-130) | |
| Wild type (PSY580) | N | N | N | N |
| srp1-31 (PSY688) | N | N | N | N |
| kap95-4 (PSY1103) | N | N/C | C/N | N |
| Δkap104 (PSY964) | C/N | N | N | N |
| Δkap114 (PSY1784) | N | N | ND | N |
| pse1-1 Δkap123 (PSY1042) | N | N | N | N |
| mtr10-1 (PSY1133) | N | N | ND | N |
| Δsxml (PSY1200) | C/N | N | N | N |
| Δnmd5 (PSY1199) | C/N | C/N | N | N |
| Δmsn5 (PSY1138) | N | N | ND | N |
| xpol-1 (PSY1105) | N | N | ND | N |
| csel-1 (PSY1040) | N | N | ND | N |
a
Strains are listed according to the original nomenclature given in parentheses.
b
Abbreviations: N, reporter proteins detected exclusively in nuclei; N/C and C/N, nucleocytoplasmic distribution of fusions where the GFP signal detected in nuclei appeared to be stronger than that in the cytoplasm, or vice versa, respectively; ND, experiment not done.