Figure 5. PTEN-ΔT CD4+ CD25+ CD45RBlo cells remain hypoproliferative to TCR stimulation.
(A) Purified PTEN-ΔT or wild-type CD4+CD25+CD45RBlo cells were CFSE labeled and stimulated with plate-bound anti-CD3 (5 μg/ml) for 72 hours. (B) Supernatants were harvested under the conditions described above after 24 hours stimulation, and levels of IL-2 were determined by ELISA. Data shown represent the mean ± SD of triplicate samples. Data are representative of 3 separate experiments. (C) Purified PTEN-ΔT or wild-type CD4+CD25+CD45RBlo cells were stimulated with IL-2 (100 U/ml), irradiated APCs, and varying doses of anti-CD3 (2C11) as shown for 72 hours. Tritiated thymidine was added to cultures for the final 16 hours before harvesting. Data shown represent the mean ± SD of triplicate samples. WT25+, CD4+CD25+CD45RBlo cells from wild-type mice; ΔT25+, CD4+CD25+CD45RBlo cells from PTEN-ΔT mice; WT25–, CD4+CD25–CD45RBhi cells from wild-type mice; ΔT25–, CD4+CD25–CD45RBhi cells from PTEN-ΔT mice.