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. 1981 Oct;44(2):409–417.

Delayed hypersensitivity to Trypanosoma cruzi in mice: specific suppressor cells in chronic infection.

M T Scott
PMCID: PMC1555205  PMID: 6457792

Abstract

In mice chronically infected with Trypanosoma cruzi there are antigen-specific suppressor cells which inhibit the development of delayed hypersensitivity (DTH) to T. cruzi antigen but not to an unrelated antigen, keyhole limpet haemocyanin (KLH). This was shown by comparing the 24 hr footpad reactivity, elicited by injection of soluble T. cruzi antigen, of infected mice with that of mice immunized with killed T. cruzi antigen after pretreatment with cyclophosphamide. In the latter, 24 hr footpad swelling represented a DTH reaction in that the cellular infiltrate was predominantly mononuclear and the reactivity could be transferred to normal recipients by lymphoid cells but not by serum. Chronically-infected mice also developed 24 hr footpad swelling but the fact that this was undiminished from an earlier 3 hr reaction and could not be transferred to normal recipients by either local or systemic injection of cells, as well as the histological features, all implied that it represented a prolonged Arthus reaction. The absence or minimal levels of specific DTH detectable in chronic T. cruzi infected mice was accompanied by the presence in their spleens of cells which specifically suppressed the generation of DTH to T. cruzi in normal mice. Suppressor cell activity was radioresistant (10 Gy/1000 rad) and T-cell mediated as defined by significantly decreased and increased suppression following anti-Thy 1.2 serum treatment and nylon wool fractionation, respectively. The ability of chronic T. cruzi mice to develop DTH to an unrelated antigen KLH was unimpaired.

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Selected References

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  1. BONE G. J., PARENT G. Stearic acid, an essential growth factor for Trypanosoma cruzi. J Gen Microbiol. 1963 May;31:261–266. doi: 10.1099/00221287-31-2-261. [DOI] [PubMed] [Google Scholar]
  2. Corsini A. C., Oliveira O. L., Costa M. G. Unimpaired delayed type hypersensitivity reactions in mice infected with Trypanosoma cruzi strain Y. Z Parasitenkd. 1980 Jan;61(2):179–185. doi: 10.1007/BF00925463. [DOI] [PubMed] [Google Scholar]
  3. Crowle A. J. Delayed hypersensitivity in the mouse. Adv Immunol. 1975;20:197–264. doi: 10.1016/s0065-2776(08)60209-6. [DOI] [PubMed] [Google Scholar]
  4. Franco M. F., Morley J. Use of 125I-labelled albumin for the detection and measurement of delayed-hypersensitivity reactions in the mouse. J Immunol Methods. 1976;11(1):7–14. doi: 10.1016/0022-1759(76)90013-2. [DOI] [PubMed] [Google Scholar]
  5. Howard J. G., Hale C., Liew F. Y. Immunological regulation of experimental cutaneous leishmaniasis. III. Nature and significance of specific suppression of cell-mediated immunity in mice highly susceptible to Leishmania tropica. J Exp Med. 1980 Sep 1;152(3):594–607. doi: 10.1084/jem.152.3.594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  7. Kierszenbaum F., Howard J. G. Mechanisms of resistance against experimental Trypanosoma cruzi infection: the importance of antibodies and antibody-forming capacity in the Biozzi high and low responder mice. J Immunol. 1976 May;116(5):1208–1211. [PubMed] [Google Scholar]
  8. Krettli A. U., Brener Z. Protective effects of specific antibodies in Trypanosoma cruzi infections. J Immunol. 1976 Mar;116(3):755–760. [PubMed] [Google Scholar]
  9. Madeira E. D., de Andrade A. F., Bunn-Moreno M. M., Barcinski M. Antibody-dependent cellular cytotoxicity of Trypanosoma cruzi: characterization of the effector cell from normal human blood. Infect Immun. 1979 Jul;25(1):34–38. doi: 10.1128/iai.25.1.34-38.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McHardy N. Passive immunization of mice against Trypanosoma cruzi using convalescent mouse serum. Tropenmed Parasitol. 1977 Jun;28(2):195–201. [PubMed] [Google Scholar]
  11. Neal R. A., Miles R. A. The number of trypomastigotes of Trypanosoma cruzi, required to infect Rhodnius prolixus. Rev Inst Med Trop Sao Paulo. 1977 May-Jun;19(3):177–181. [PubMed] [Google Scholar]
  12. Neal R. A., Miles R. A. The sensitivity of culture methods to detect experimental infections of Trypanosoma cruzi and comparison with xenodiagnosis. Rev Inst Med Trop Sao Paulo. 1977 May-Jun;19(3):170–176. [PubMed] [Google Scholar]
  13. Olabuenaga S. E., Cardoni R. L., Segura E. L., Riera N. E., de Bracco M. M. Antibody-dependent cytolysis of Trypanosoma cruzi by human polymorphonuclear leukocytes. Cell Immunol. 1979 Jun;45(1):85–93. doi: 10.1016/0008-8749(79)90364-2. [DOI] [PubMed] [Google Scholar]
  14. Poulter L. W., Lefford M. J. Development of delayed-type hypersensitivity during Mycobacterium lepraemurium infection in mice. Infect Immun. 1977 Aug;17(2):439–446. doi: 10.1128/iai.17.2.439-446.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ramos C., Schädtler-Siwon I., Ortiz-Ortiz L. Suppressor cells present in the spleens of Trypanosoma cruzi-infected mice. J Immunol. 1979 Apr;122(4):1243–1247. [PubMed] [Google Scholar]
  16. Ramshaw I. A., Bretscher P. A., Parish C. R. Regulation of the immune response. I. Suppression of delayed-type hypersensitivity by T cells from mice expressing humoral immunity. Eur J Immunol. 1976 Oct;6(10):674–679. doi: 10.1002/eji.1830061003. [DOI] [PubMed] [Google Scholar]
  17. Reed S. G., Larson C. L., Speer C. A. Contact sensitivity responses in mice infected with Trypanosoma cruzi. Infect Immun. 1978 Nov;22(2):548–554. doi: 10.1128/iai.22.2.548-554.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Reed S. G., Larson C. L., Speer C. A. Suppression of cell-mediated immunity in experimental Chagas' disease. Z Parasitenkd. 1977 Jun 3;52(1):11–17. doi: 10.1007/BF00380553. [DOI] [PubMed] [Google Scholar]
  19. Reis A. P., Chiari C. A., Tanus R., Andrade I. M. Cellular immunity to Trypanosoma cruzi infection in mice. Rev Inst Med Trop Sao Paulo. 1976 Nov-Dec;18(6):422–426. [PubMed] [Google Scholar]
  20. Rowland E. C., Kuhn R. E. Suppression of anamnestic cellular responses during experimental American trypanosomiasis. J Parasitol. 1978 Aug;64(4):741–742. [PubMed] [Google Scholar]
  21. Rowland E. C., Kuhn R. E. Suppression of cellular responses in mice during Trypanosoma cruzi infections. Infect Immun. 1978 May;20(2):393–397. doi: 10.1128/iai.20.2.393-397.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Santos-Buch C. A. American trypanosomiasis: Chagas' disease. Int Rev Exp Pathol. 1979;19:63–100. [PubMed] [Google Scholar]
  23. Seah S. Delayed hypersensitivity in Trypanosoma cruzi infection. Nature. 1970 Mar 28;225(5239):1256–1257. doi: 10.1038/2251256a0. [DOI] [PubMed] [Google Scholar]
  24. Shand F. L. The immunopharmacology of cyclophosphamide. Int J Immunopharmacol. 1979;1(3):165–171. doi: 10.1016/0192-0561(79)90038-9. [DOI] [PubMed] [Google Scholar]
  25. Teixeira A. R., Santos-Buch C. A. The immunology of experimental Chagas' disease. II. Delayed hypersensitivity to Trypanosoma cruzi antigens. Immunology. 1975 Mar;28(3):401–410. [PMC free article] [PubMed] [Google Scholar]
  26. Teixeira A. R., Teixeira G., Macêdo V., Prata A. Acquired cell-mediated immunodepression in acute Chagas' disease. J Clin Invest. 1978 Dec;62(6):1132–1141. doi: 10.1172/JCI109232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Teixeira A. R., Teixeira G., Macêdo V., Prata A. Trypanosoma cruzi-sensitized T-lymphocyte mediated 51CR release from human heart cells in Chagas' disease. Am J Trop Med Hyg. 1978 Nov;27(6):1097–1107. doi: 10.4269/ajtmh.1978.27.1097. [DOI] [PubMed] [Google Scholar]
  28. Toledo Barros M. A., Amato Neto V., Mendes E., Mota I. In vitro cellular immunity in Chagas' disease. Clin Exp Immunol. 1979 Nov;38(2):376–380. [PMC free article] [PubMed] [Google Scholar]

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