Fig. 4.
Transcription from a TATA-less, DPE-containing promoter requires TAF1 and TAF4. (A) Diagrams of the reporter constructs used to transfect S2 cells. (Upper) WT MtnA promoter driving luciferase expression. It contains a TATA box (TATAAAA) and an initiator (TCAGTT), but no DPE (AATCATC starting at position +28). (Lower) WT MtnA promoter with a mutated TATA box (GCGCCCC) and a DPE (AGACGTG) inserted at position +28 from the transcription start site. (B) S2 cells were either left untreated (NT) or treated with dsRNA directed against TAF1, TAF4, or TAF5 and transfected with either the WT MtnA-luc reporter or the mMtnA+DPE-luc reporter and actin-Renilla to control for transfection efficiency. After 3 days, the transfected cells were treated with copper to induce transcription. Six hours later, luciferase expression was determined, normalized to Renilla expression, and plotted as fraction of untreated luminescence.