Figure 2.
Effect of antagonists SR141716A, SR144528 and capsazepine on anandamide accumulation in PC-3 cells. PC-3 cells were preincubated at 37°C in the presence of the antagonists (50 μM) and incubated with 100 nM anandamide. The results represent specific anandamide accumulation at 37°C corrected for nonspecific binding at 4°C and are expressed as mean±s.e. of two independent experiments performed in triplicate.