Table 1.
Antibodies to dsDNA, Nucleosomes, and α -Actinin and Their Relationship to Proteinuria in B/W Mice Studied over 33 Weeks
| B/W mouse no. | Age (weeks) at production of
|
Age at proteinuria* | Antibody pattern prior to proteinuria | Pattern example (see Figure 1)† | ||
|---|---|---|---|---|---|---|
| Anti-dsDNA | Anti-nuc | Anti-α-actinin | ||||
| 2 | 30 | 30 | 30 | 2 (+3) | Decreasing | D |
| 3 | 30 | 30 | nd‡ | 32 (+3) | Stable | E |
| 4 | 21 | 33 | nd | 29 (+3) | Stable | E |
| 6 | 17 | 30 | nd | 26 (+3) | Increasing | F |
| 9 | 21 | 17 | nd | 24 (+3) | Low antibody levels | – |
| 10 | 30 | 30 | nd | 32 (+3) | Stable | E |
| 11 | 30 | 21 | nd | 32 (+3) | Stable | E |
| 12 | 17 | 33 | 33 | 32 (+2) | Increasing | F |
| 13 | 30 | 30 | nd | 32 (+3) | Stable | E |
| 15 | 30 | 30 | 30 | (+1) | No proteinuria over 33 weeks | G |
| 16 | 21 | 21 | nd | 33 (+4) | Decreasing | D |
| 18 | 30 | 21 | nd | 32 (+3) | Increasing | F |
| 19 | 30 | 17 | nd | 17 (+1) | Increasing | F |
| 20 | 21 | 30 | nd | 32 (+2) | Decreasing | D |
| Mean (±SD) | 25.6 (±5.4) | 26.6 (±5.8) | Not determined | 30.5 (±2.8) | ||
*
Proteinuria was determined by stix from Bayer Diagnostics (Bridgend, United Kingdom): 0–1+, ≤0.3 g/l; 2+, ≤1 g/l; 3+, ≤3 g/l; 4+, ≥20 g/l.
†
The antibody profiles, as determined by ELISA using 1/100 serum dilution on serial serum samples, possessed four principally different profiles linked to development of proteinuria, as demonstrated in Figure 1, D–G.
‡
nd, not detected.