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. 2003 Jun;2(3):599–608. doi: 10.1128/EC.2.3.599-608.2003

TABLE 2.

Summed fractional labelings obtained with the reference strain in continuous cultivations at dilution rate of 0.1 h−1 for different acetate fractionsa

Compound analyzed Mol wt of unlabeled isotopomer Biosynthetic precursor(s) C atoms from biosynthetic precursor(s) present in fragment Sum of fractional labelings (%) of C atoms in a fragment with the following acetate fraction (C-mol/C-mol of substrate) and labeled substrate used:
0b [1-13C]glucose 0.04, [1,2-13C]acetate 0.46c, [1,2-13C]acetate 0.90d, [13C6]glucose
Glc 331 Glc 6P 1, 2, 3, 4, 5, 6 91.0 6.9 277.9
Gly 175e 3-PG 1, 2 5.9 3.2 10.2 21.3
144f 3-PG 1, 2 6.2 3.1 11.4
85f 3-PG 2 3.7 1.3 5.1 9.6
Ser 175e 3-PG 1, 2 3.1 3.1 5.9 34.0
132e 3-PG 2, 3 33.8 2.4 5.6 31.5
Phe 143f PEP 1, 2 3.0 2.2 4.3 25.3
192e PEP + E4P 2, 2, 3, 3 + 1, 2, 3, 4 90.6 9.4 14.9 212.1
Ala 116e Pyr 2, 3 36.0 3.0 12.5 14.9
99f Pyr 2, 3 36.0 3.2 12.5 15.1
158f Pyr 1, 2, 3 38.5 4.7 18.3 21.1
Val 144e Pyr 2, 2, 3, 3 73.3 6.5 27.3 21.2
143f Pyr 1, 2 6.9 2.9 13.8 10.2
127f Pyr 2, 2, 3, 3 73.1 6.3 26.8 20.1
186f Pyr 1, 2, 2, 3, 3 74.9 7.9 33.3 26.5
Leu 158e Pyr + AcCoA 2, 2, 3, 3 + 2 106.1 15.6 42.3 19.8
Asp 188e OAA 2, 3, 4 57.1 11.4 39.8 7.2
115f OAA 2 12.0 3.4 11.7 2.5
216f OAA 1, 2, 3, 4 64.4 14.3 52.8 10.0
Thr 175e OAA 1, 2 17.8 6.8 25.0 5.0
146e OAA 2, 3, 4 54.5 12.1 35.7 8.2
Ile 158e OAA + Pyr 2, 3, 4 + 2, 3 93.7 14.5 53.4 16.9
Pro 142e α-KG 2, 3, 4, 5 85.5 18.8 58.3 6.9
Glu 143f α-KG 1, 2 39.9 10.5 28.4 6.5
230f α-KG 1, 2, 3, 4, 5 100.1 23.7 72.1 11.6
Lys 156e α-KG + AcCoA 2, 3, 4, 5 + 2 117.2 48.5 75.6 8.4
a

A naturally labeled fragment with n carbon atoms will be labeled n.n%. The biosynthetic precursors indicated are for growth on glucose and ammonia; for some amino acids, the biosynthetic pathways may change for growth on acetate. Abbreviations: 3-PG, 3-phosphoglycerate; Pyr, pyruvate; AcCoA, acetyl-CoA; OAA, oxaloacetate; α-KG, α-ketoglutarate; PEP, phosphoenolpyruvate; E4P, erythrose-4-phosphate; Glc, glucose; Glc 6P, glucose-6-phosphate.

b

Data are from reference 15.

c

Only 20% of the acetate fed to the culture was [1,2-13C] acetate.

d

pH6.

e

Amino acid analyzed as N-ethoxycarbonyl amino acid ethylester.

f

Amino acid analyzed as N-(N,N-dimethylaminomethylene) amino acid methylester.