FIG. 6.
Mitogen responsiveness of T cells derived from Mek2−/− mice. (A) Total thymocytes from wild-type and Mek2−/− mice (2 × 105 cells) were cultured in medium (ctl) or in the presence of IL-2, anti-CD3ɛ, or anti-CD28 alone or in combination in 96-well plates. Cells were stimulated with the indicated concentrations of mitogens, 10 U of IL-2/ml, anti-CD3ɛ (plated at 10 μg/ml), or anti-CD28 (plated at 10 μg/ml) for 72 h. The results shown are the means ± the standard errors of the means of results for nine different experiments done in triplicate. No statistically significant difference was observed. (B) Total T cells from wild-type and Mek2−/− spleens (2 × 105 cells) were cultured in the presence of various concentrations of anti-CD3ɛ (plated at 0.1 to 10 μg/ml). The results shown are the means ± the standard errors of the means of results from six different experiments done in triplicate. No statistically significant difference was observed. CPM, counts per minute.