TABLE 1.
CHIP enhances and Hsp40 reduces the amount of nascent polypeptide chains that coimmunoprecipitate with Hsp70 in the presence of an ATP-regenerating systema
| Transfection | Relative amt of nascent chains coimmunoprecipitating with Hsp70 ± SEM
|
|
|---|---|---|
| Without ATP (apyrase) | With ATP | |
| None (control) | 1.00 ± 0.08 | 1.00 ± 0.20 |
| CHIP | 1.04 ± 0.14 | 3.21 ± 0.63 |
| Hsp40 | 1.15 ± 0.21 | 0.63 ± 0.11 |
| Bag-1 | 0.30 ± 0.18 | 0.80 ± 0.16 |
a
CHO cells were transfected with Myc-tagged Hsc70 alone or together with CHIP, Hsp40, or Bag-1 and pulse-labeled with [35S]methionine at 48 h after transfection. Lysates were prepared in the presence of apyrase or an ATP-regenerating (ATP) system. Immunoprecipitation was performed with Myc-Hsp70, the precipitated material was run on SDS-PAGE, and the amount of radioactivity was quantified with a phosphorimager. Averaged data from three to four independent experiments are provided.