Table 1. SNPs and primers for the haplotyping assays.
SNP ID | Chromosome position | PCR primer 1 | PCR primer 2 | Extension primer | Terminator mix* |
---|---|---|---|---|---|
rs289741 | 47282625 | TCTACCAGCTTGGCTCCCTC | AAGTCCATCAGCAGCAGCAG | GGGAGTCAGCCCAGCTC | ACT |
rs289742 | 47282337 | ACTGGTGAGACAATCCCTTC | CCACTGGCATTAAAGTGCTG | AGCCACAGAAGAAGGACTCC | ACT |
rs289744 | 47281997 | TACCAGAAACCAGACCTCTG | AGTGCTGGACAGAAAGTGAG | TGAGGATGGTGGGAGGG | ACT |
rs289744† | 47281997 | TCTACCAGAAACCAGACCTC | AGTGCTGGACAGAAAGTGAG | ACCTCTGAGGGCCCCTTAC | CG |
rs2228667† | 47282820 | CTCGAGTGATAATCTCAGGG | AGGTAGTGTTTACAGCCCTC | TGATGATGTCGAAGAGGCTCATG | CG |
rs5882† | 47284007 | TTACGAGACATGACCTCAGG | GCATTTGATTGGCAGAGCAG | CTGCAGGAAGCTCTGGATG | CG |
rs5882‡ | 47284007 | GCATTTGATTGGCAGAGCAG | TTACGAGACATGACCTCAGG | AGAGCAGCTCCGAGTCC | ACT |
rs5880‡ | 47285008 | GCAGCACATACTGGAAATCC | TTTCTCTCCCCAGGATATCG | GCTTTTTCTTAGAATAGGAGG | ACT |
rs5881† | 47288087 | AGATCTTGGGCATCTTGAGG | ACCCCTGTCTTCCACAGGTT | TGGGCCTGGCTGGGGAAGC | CG |
rs5881‡ | 47288087 | ACCCCTGTCTTCCACAGGTT | AGATCTTGGGCATCTTGAGG | TGTCTTCCACAGGTTGTCGGC | ACT |
rs291044† | 47288647 | GTAAAACTGCAGCTGAGGAG | TGACTAGGTCAGGTCCCCTC | GGAGTATTTAAAGGAGAGACACA CTAG | CG |
rs291044‡ | 47288647 | TGACTAGGTCAGGTCCCCTC | GTAAAACTGCAGCTGAGGAG | CCCTCGTGCCACAGCCT | ACT |
rs2033254‡ | 47290114 | GGACATCAAAGGAACAGGAC | ACTCACAATATTGGGCAGGC | CAAGGGGCTAAGGGAGAAG | ACT |
IGR2198a_1§ | 506266¶ | GGGTTGCATGAGCATTAAGT | CACATCAAGGATAAGACTGC | ATCTCTTCAGTAGACGAAC | AC |
IGR2175a_2§ | 495082¶ | TGGCCTTGATTCAAACCCTG | AGATGAAGGAAATCCCAAGG | TGCCACTAACATACATAGTAAC | AC |
IGR2150a_1§ | 482171¶ | CCTTGGCTTGATAGTCAAAC | ATTTGGAGGAGTGCAGAGAG | AGTCAAACTCTCACCAC | AC |
ACT mix is ddATP/ddCTP/ddTTP/dGTP in which dd indicates the 2′,3′-dideoxynucleotide. Similarly, CG mix is ddCTP/ddGTP/dATP/dTTP.
Multiplex assay 1.
Multiplex assay 2.
SNP ID from Daly et al. (23); see also www-genome.wi.mit.edu/humgen/IBD5/haplodata.html.
Position of SNP from Daly et al. (23).