Table 1.
Yeast two-hybrid expression vector constructs
| pAS/pACT-hNup153.1-224: Both constructs encode spacer-free fusions between Gal4 domains and hNup153 aa1-244, followed by vector-derived C-terminal residues ARPAAKLIPGEFLMIYDFYY(pAS-end1), or AREJYES (pACT-end1). |
| pAS/pACT-hNup153.228-439: MGS, hNup153 aa228-439, plus pAS-end1, or pACT-end1. |
| pAS/pACT-hNup153.337-611: MGS, hNup153 aa337-611, plus pAS-end1, or pACT-end1. |
| pAS/pACT-hNup153.228-611: MEAGS, hNup153 aa228-611, plus pAS-end1, or pACT-end1. |
| pAS/pACT-rNup98.1-339: MEAEFPGIRI, rNup98 aa1-339, plus pAS-end1, and MEAPGIRI, rNup98 aa1-339, plus pACT-end1. |
| pAS/pACT-rNup98.316-844: rNup98 aa316-844, plus REIRRPAAKLIPGEFLMIYDFYY (pAS-end2), or REIYES (pACT-end2). |
| pAS-rNup98.2-844: MEAEFPGIRIGAFMST, rNup98 aa2-844, plus pAS-end2, and MEAPGIRIGAFMST, rNup98 aa2-844, plus pACT-end2. |
| pACT-rNup196.316-883: rNup196 aa316-883, plus VRSQEEAESHRGSVERSMNRRY. |
| pAS/pACT-hNup196.864-1261: MEAEFPGIRIPGS (for pAS2-1) or MEAPGIRIPGS (for pACT2), hNup196 aa864-1216, plus LTVDLGRSMNRRY. |
| pAS/pACT-rNup196.1158-1599: rNup196 aa1158-1599, plus RRPAAKLIPGEFLMIYDFYY for pAS2-1, and rNup196 aa1158-1599, plus RIR, and pACT-end1. |
| pAS/pACT-hNup93.1-312: MRIP, hNup93 aa1-312, plus pAS-end1, and MEAPGIP, hNup93 aa1-312, plus pACT-end1. |
| pAS/pACT-hNup93.226-603: hNup93 aa226-603, plus VRSQEEAESHR, and GSVDLQPS for pAS2-1, and GSEFELERSMNRRY for pACT2. |
| pAS/pACT-hNup93.226-819: hNup93 aa226-819. |
| pAS/pACT-hNup107.1-146: MEAEFPGIRI, hNup107 aa1-146, plus pAS-end1, and MEAPGIRI, hNup107 aa1-146, plus pACT-end1. |
| pACT-hNup107.101-606: hNup107 aa101-606, plus AKLIPGEFLMIYDFYY. |
| pAS/pACT-hNup107.101-769: hNup107 aa101-769, plus RGSVDLQPS for pAS2-1, and SSRDL for pACT2. |
| pAS-hNup107.101-925: hNup107 aa101-925. |
| pAS/pACT-hNup205.1-718: hNup205 aa1-718, plus S, and TCSQANSGRISYDL (pAS-end3), and RDL for pACT2. |
| pAS-hNup205.350-1907: ME, hNup205 aa350-1907, plus pAS-end3. |
| pAS/pACT-hNup205.1-1907: hNup205 aa1-1907, plus pAS-end3, and DL for pACT2. |
| pACT-hNup205.350-718: hNup205 aa350-718, plus RDL. |
| pAS-hNup205.1-1254: hNup205 aa1-1254, plus PS. |
| pAS-hNup205.350-1254: hNup205 aa350-1254, plus PS. |
| pAS/pACT-hNup50.1-126: MRIR, hNup50 aa1-126, plus pAS-end1, and MEAPGIRI, hNup50 aa1-126, plus pACT-end1. |
| pACT-hNup50.29-208: hNup50 aa29-208, plus RGSVDLQPS for pAS2-1, and SSRDL for pACT2. |
| pAS-hNup50.29-469: hNup50 aa29-469. |
| pACT-hNup160.1-301: MEAPGIP, hNup160 aa1-301, and pACT-end1. |
| pAS-hNup160.238-1255: hNup160 aa238-1255, plus YGPKKKRKVTGDPS, and pAS-end3. |
| pAS-hNup160.238-764: hNup160 aa238-765, plus AYGPKKKRKVTGDPS, and pAS-end3. |