Table 2.
Bacterial expression vector constructs
| pGEX.hTpr.76/172-651: Encodes an N-terminal GST-tag, followed by LEVLFQGP (Pre Scission recognition site, cleavage site between residues Q and G), spacer LGSMADTFLEH, hTpr aa1-76, hTpr aa172-651, plus TGDPNSSGRIVTD. |
| pGEX.hTpr.76/172-651DD and PP: As pGEX.hTpr.76/172-651 but with aa substitution mutations L458D and M489D or L458P and M489P within the hTpr aa sequence segment. |
| pGEX.hNup153.228-611: N-terminal GST-tag, followed by LEVLFQGP, spacer LGS, hNup153 aa228-611, plus SSGRIVTD. |