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. 2006 Sep 20;44(11):3980–3988. doi: 10.1128/JCM.00312-06

TABLE 2.

Primers used in this study

Primera Sequence (5′-3′) Specificity Annealing temp (°C) DGGE gradient (%)c Reference
968f-GCb AAC GCG AAG AAC CTT AC Bacterial V6-V8 region 56 30-55 40
1401r CGG TGT GTA CAA GAC CC Bacterial V6-V8 region 56 40
Bif164f GGG TGG TAA TGC CGG ATG Bifidobacterium 66 45-55 22
Bif662r-GCb CCA CCG TTA CAC CGG GAA Bifidobacterium 66 22
Lac1f AGC AGT AGG GAA TCT TCC A LAB 61 30-55 65
Lac2r-GCb ATT YCA CCG CTA CAC ATG LAB 61 65
g-Bfra-F-GCb ATA GCC TTT CGA AAG RAA GAT Bacteroides fragilis group 50 30-50 33
g-Bfra-R CCA GTA TCA ACT GCA ATT TTA Bacteroides fragilis group 50 33
Sg-Clept-F GCA CAA GCA GTG GAG T Clostridium leptum subgroup 50 30-50 34
sg-Clept-R3-GCb CTT CCT CCG TTT TGT CAA Clostridium leptum subgroup 50 34
g-Ccoc-F-GCb AAA TGA CGG TAC CTG ACT AA Clostridium coccoidessubgroup 50 35-50 33
g-Ccoc-R CTT TGA GTT TCA TTC TTG CGA A Clostridium coccoides subgroup 50 33
a

f (forward) and r (reverse) indicate the orientation of the primers with respect to the 16S rRNA gene sequence.

b

GC indicates a 40-bp GC-rich sequence attached to the 5′ end of the primer: 5′-CCCGCCGCGCCCCGCGCCCGTCCCGCCGCCCCCGCCCG-3′.

c

Denaturing acrylamide of 100% was defined as 7 M urea and 40% (vol/vol) formamide.