Table 1.
Effect of co-activation of μ opioid and CB1 cannabinoid receptors on neurite outgrowth
| |
% cells with neurites with drugs at |
||
|---|---|---|---|
| 1 × 10−7 M | 1 × 10−6 M | 1 × 10−5 M | |
| Morphine |
22.7±1.5** |
27.3±1.2** |
32.0±1.5** |
| DAMGO |
37.7±0.9** |
41.7±1.0** |
43.3±0.5** |
| Hu-210 |
38.0±1.2** |
44.7±0.9** |
45.3±1.7** |
| Morphine+ |
|
|
|
| Hu-210 |
16.0±1.3**,++ |
12.4±0.3**,++ |
8.7±0.3**,++ |
| DAMGO+ |
|
|
|
| Hu-210 | 13.0±0.9**,++ | 9.9±0.3**,++ | 7.2±0.7**,++ |
Neuro 2A cells stably expressing Flag-tagged μ opioid receptors were treated with different concentrations of morphine, DAMGO (μ agonists), Hu-210 (CB1 agonist) or combination of these drugs as described in Methods. Control cells were not exposed to any drug treatment (∼2.3±0.33% cells had neurites). Cells were scored as positive for neurite outgrowth when the length of the neurite was more than twice the diameter of the cell. Results are the mean±s.e.m. (n=6).
**
P<0.001 versus control;
++
P<0.001 versus morphine/DAMGO/Hu-210; one-way ANOVA (n=6).