Table 1. Detection of M. tuberculosis DNA in adipose tissue from Mexican autopsy samplesa .
Patients' characteristics | Presence of mycobacterial DNA (PRAT/PAT/PNAT)b | ||||
Patient # | Sex | Age at death (Years) | Cause of death | In situ PCR | Conventional PCR |
1 | F | 69 | Sepsis | −/−/− | −/−/− |
2 | F | 18 | Sepsis | −/−/− | −/−/− |
3 | M | 35 | Hypovolemic shock | +/−/+ | +/−/− |
4 | M | 64 | Lung cancer | −/+/− | −/+/− |
5 | M | 42 | Malignant glioma | −/−/− | −/−/− |
6 | F | 57 | Sepsis | −/−/− | −/−/− |
7 | M | 89 | Pneumonia | +/+/− | −/−/− |
8 | M | 29 | Myeloblastic leukemia | −/−/− | −/−/− |
9 | F | 29 | Kidney cancer | −/−/+ | −/−/− |
10 | M | 80 | Emphysema | −/−/+ | −/−/+ |
11 | F | 29 | Hypovolemic shock | −/−/− | −/−/− |
12 | F | 83 | Stroke | +/−/+ | −/−/− |
13 | F | 22 | Diabetes | −/−/− | −/−/− |
14 | M | 36 | Stroke | −/−/− | −/−/− |
15 | F | 70 | Breast cancer | −/−/− | −/−/− |
16 | F | 50 | Heart failure | −/−/− | −/−/− |
17 | M | 76 | Heart failure | −/−/− | −/−/− |
18 | F | 59 | Chronic kidney failure | −/−/− | −/−/− |
19 | F | 14 | Sepsis | −/−/− | −/−/− |
Nine samples were positive for mycobacterial DNA by in-situ PCR, and three by conventional PCR. Three of these nine were positive by both methods. Six samples were positive only by in-situ PCR, and no sample was positive only by conventional PCR. Thus 51 of 57 samples were concordant by the two methods
PRAT, peri-renal adipose tissue; PAT, peritoneal adipose tissue; PNAT, peri-nodal adipose tissue