Figure 5.
Release of exovesicles from iDCs in the vicinity of mDCs. A: LSM image of DiO-labeled iDCs (green) co-cultured with DiI-labeled LPS-stimulated DCs (red) in collagen matrix for 24 hours. In A′, incorporated red material can be observed (arrows). A: Three-dimensional reconstruction; A′: xy- and xz-projections from the same data set; yellow arrowheads mark the position of projections. B: Diagram showing total amount of external objects released by iDCs in the co-culture of nonstimulated DCs (left panels) or in the co-culture with LPS-stimulated DCs (right panels). Data are expressed as mean ± SD of two experiments with LSM scanning of 8 to 10 cells each. The asterisk represents a statistically significant difference (P < 0.001) between LPS-treated and control groups.