Table 3.
The frequency of immunoglobulin-switched mature B cells (B220high μ−) in bone marrow after E2 treatment
| % B220high μ− bone marrow cells | ||
|---|---|---|
| Controls | 30 μg E2/kg | |
| Wild-type mice | ||
| ERα+ ERβ+ | 3·2 ± 0·4 | 18·6 ± 3·7* |
| BERKO mice | ||
| ERα+ ERβ− | 2·9 ± 0·6 | 13·1 ± 1·2** |
| ERKO mice | ||
| ERα− ERβ+ | 3·4 ± 0·5 | 6·7 ± 0·9 |
| DERKO mice | ||
| ERα− ERβ− | 4·2 ± 0·3 | 5·0 ± 0·6 |
| anova ERα | ††† | |
| anova ERβ | ns | |
Male mice of all four ER genotypes were castrated and treated for 2·5 weeks with subcutaneous doses of E2 or olive oil as controls. Frequency of B220+ cells of B220high μ− phenotype was obtained by use of flow cytometry. All groups consisted of four to six mice. Mean ±SEM.
*
P < 0·05.
**
P < 0·01 (Mann–Whitney: controls versus E2).
†††
P < 0·001 (anova: ERα+ versus ERα−).
ns, not significant.