Figure 5.
Bak-dependent release of cytochrome c during Ngo- and cisplatin-induced apoptosis. (A) HeLa cells permanently expressing shBak or shBax were infected and cytochrome c release upon infection was visualized by immunofluorescence using anti-cytochrome c antibody. The GFP-expression derived from the vector used for shRNA transcription. (B) Cisplatin-induced cytochrome c release was monitored by co-staining the cells with anticytochrome c antibody and Hoechst 33342. Arrows indicate cells showing a release of cytochrome c. (C–D) Additionally the release of cytochrome c in shBak and shBax cells upon infection and cisplatin treatment was monitored by the use of anticytochrome c antibody in cytosolic extracts upon subcellular fractionation. ShBak cells showed no release of Cytochrome c, whereas shBax and controls released Cytochrome c upon cisplatin treatment or Ngo infection.