Table 3.
Steady-state activation properties of the wild-type, multiple M-to-L, and M-to-E mutant channels in the virtual absence of Ca2+
| Channel | Pre-V0.5 (mV) | Post-V0.5 (mV) | ΔV0.5 (mV) | Pre-Qapp (e) | Post-Qapp (e) | ΔQapp (e) |
|---|---|---|---|---|---|---|
| WT | 171.9 ± 4.5 | 140.6 ± 6.1 | −31.3 ± 3.3 | 1.1 ± 0.16 | 0.84 ± 0.09 | −0.25 ± 0.07 |
| Triple Met | 161.1 ± 4.8 | 158.7 ± 4.3 | −2.4 ± 1.3 | 1.1 ± 0.07 | 0.90 ± 0.06 | −0.17 ± 0.09 |
| M536L–M712L | 177.9 ± 5.6 | 144.0 ± 8.8 | −33.9 ± 3.3 | 0.7 ± 0.05 | 0.61 ± 0.02 | −0.07 ± 0.07 |
| M536L–M739L | 166.5 ± 6.6 | 141.8 ± 5.4 | −24.8 ± 2.7 | 1.1 ± 0.01 | 0.97 ± 0.01 | −0.11 ± 0.003 |
| M712L–M739L | 171.5 ± 6.0 | 131.8 ± 4.4 | −39.8 ± 7.9 | 1.0 ± 0.15 | 0.90 ± 0.09 | −0.15 ± 0.10 |
| M460L–M712L–M739L | 164.0 ± 2.8 | 125.3 ± 3.1 | −38.6 ± 2.5 | 1.2 ± 0.10 | 0.97 ± 0.12 | −0.28 ± 0.17 |
| M652L–M712L–M739L | 123.8 ± 4.9 | 90.18 ± 3.3 | −33.6 ± 6.9 | 1.3 ± 0.04 | 1.03 ± 0.05 | −0.28 ± 0.005 |
| M712L–M739L–M851L | 152.7 ± 3.4 | 121.6 ± 4.4 | −31.1 ± 1.1 | 1.2 ± 0.11 | 1.01 ± 0.11 | −0.22 ± 0.029 |
| M563E | 226.7 ± 4.4 | 194.1 ± 7.8 | −32.7 ± 5.3 | 0.9 ± 0.02 | 0.77 ± 0.08 | −0.16 ± 0.059 |
| M712E | 140.7 ± 4.0 | 128.2 ± 5.0 | −12.5 ± 2.8 | 1.2 ± 0.11 | 0.91 ± 0.09 | −0.27 ± 0.058 |
| M739E | 109.6 ± 2.6 | 99.73 ± 3.1 | −9.8 ± 2.3 | 1.3 ± 0.03 | 0.95 ± 0.06 | −0.35 ± 0.04 |