Fig. 2.
TAK1 is essential for osmotic stress-induced JNK but not p38.
(A) Schematic representation of TAK1 deletion.
(B) MEF cells and TAK1Δ/Δ were subjected to osmotic stress (0.5 M NaCl) for 10 or 30 min. Cell extracts were subjected to immunoblotting with anti-phospho JNK (top panel) and anti-phospho p38 (third panel). The amounts of endogenous JNK, p38, and TAK1 are shown in the second, fourth, and bottom panels, respectively. IB, immunoblotting.
(C) Mouse keratinocytes and TAK1Δ/Δ keratinocytes were subjected to osmotic stress (0.5 M NaCl) for 10 or 30 min. Cell extracts were subjected to immunoblotting with anti-phospho JNK (top panel) and anti-phospho p38 (third panel). The amounts of endogenous JNK, p38, and TAK1 are shown in the second, fourth, and bottom panels, respectively. IB, immunoblotting.