. 1999 Sep 28;96(20):11470–11475. doi: 10.1073/pnas.96.20.11470

Table 2.

Analysis of thymocytes from TCR transgenic fetal liver chimeras

Donor	Recipient	Cells/thymus, ×10⁻⁶	Clonotype-TCR⁺CD8⁺CD4⁻ cells		HSA⁻ 2C-TCR⁺CD8⁺CD4⁻ cells
Donor	Recipient	Cells/thymus, ×10⁻⁶	% of thymus	Per thymus × 10⁻⁶	% of 2C-TCR⁺CD8⁺ cells	Per thymus × 10⁻³
2C, I⁺	β₂m⁺	14.9	18.9 (5.5)	2.91 (2.3)	19.7 (6.7)	337.9 (465.0)
2C, β₂m^−/−	β₂m^−/−	31.9	1.3 (0.3)	0.36 (0.1)	0.3 (0.3)	1.4 (2.1)
2C, I⁺	β₂m^−/−	31.0	3.6 (1.1)^¶	1.10 (0.4)^§	10.8 (4.1)^§	83.1 (55.5)^‡
					HSA⁻ CD8⁺CD4⁻ cells^*

					% of CD8⁺ cells	Per thymus × 10⁻³
2C, I⁺	K^bD^b+	19.3	19.8 (5.7)	3.94 (1.8)	21.1 (7.5)	881.8 (561)
2C, β₂m^−/−	K^bD^b−/−	35.9	1.2 (0.9)	0.38 (0.3)	5.9 (1.3)	23.4 (18)
2C, I⁺	K^bD^b−/−	37.6	3.8 (1.6)^§	1.33 (0.59)^§	12.9 (6.5)^‡	118.5 (55)^§
HY, I⁺	β₂m⁺	36.5	13.8 (4.6)	5.3 (3.6)	ND	ND
HY, β_2m^−/−	β₂m^−/−	13.3	0.1 (0.1)	0.015 (0.01)	ND	ND
HY, I⁺	β₂m^−/−	10.9	0.3 (0.2)^†	0.03 (0.02)^†	ND	ND

Numbers represent the mean values (SD) of four to nine determinations.

Significance vs. corresponding class I⁻ into class I⁻ control group.

^†

P > 0.05 (not significant);

^‡

P < 0.05;

^§

P < 0.01;

^¶

P < 0.002.

Determined from three-color staining. A few of the 2C, class I⁺ into K^bD^b−/− chimeras were analyzed by four-color staining; most of the HSA⁻CD8⁺CD4⁻ cells in these animals were 2C-TCR⁺. ND, not determined.