Fig. 1.
Flow-cytometric sorting of CD8+ CD45RA+ CD28− effector T cells. PBL from patients with cervical cancer (a), corresponding to patient CC-I, Tables 1 and 3 or from patients with pulmonary tuberculosis (b), corresponding to patient TUB-III, Tables 1 and 3 were harvested and gated based on forward and side scatter. The double-positive CD8+ CD45RA+ (in green) T cell population was determined and sorted for CD28- (marked in yellow) and CD28+ (marked in blue) staining T cells. Re-analysis of sorted T cells showed>97% purity of the CD8+ CD45RA+ CD28+ T cell subpopulations.