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Reversion analysis of H/JFH in Huh-7.5 cells
| Day postelectroporation | Reversion |
|||
|---|---|---|---|---|
| A473G | A480C | A473G + A480C | None | |
| 1–7 | 27/27 | |||
| 10 | 1/6 | 1/6 | 4/6 | |
| 11 | 1/8 | 1/8 | 6/8 | |
| 15 | 1/10 | 3/10 | 6/10 | |
| 21 | 3/13 | 9/13 | 1/13 | |
| 23 | 9/29 | 19/29 | 1/29 | |
| 38 | 3/9 | 6/9 | ||
| 41 | 1/10 | 9/10 | ||
| 50 | 1/10 | 9/10 | ||
Individual clones were sequenced from HCV RNA isolated from passaged cells, and the number of clones possessing the indicated reversions are listed.
