Table 1.
Strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| BY4741 | MAT a; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0 | Euroscarf |
| BY4742 | MAT a; his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0 | Euroscarf |
| BY4743 | MAT a/α; his3Δ1/his3Δ1; leu2Δ0/leu2Δ0; lys2Δ0; met15Δ0/met15Δ0 ura3Δ0/ura3Δ0 | Euroscarf |
| EGY021.2 | MATα; trp1; leu2; suc2-Δ9; sec21::his3; pRS315-sec21-3 | Erin Gaynor |
| GPY60 | MATα; ura3-52; leu2,3-112; his4-579; pep4::ura3; prb1; trp4-579 | Randy Schekman |
| HHY203 | MAT a; ade2-101; his3-Δ200; leu2-Δ1; lys2-801; trp1-Δ63; ura3-52; YGL051w::HIS3; YAR033w::HIS3; | This study |
| HHY204 | MATα; ade2-101; his3-Δ200; leu2-Δ1; trp1-Δ63; ura3-52; YGL089c::His12-YGL089c(URA3); YGL051w::HIS3; YAR033w::HIS3; | This study |
| HHY215 | MATα; trp1; leu2; suc2-Δ9; sec21::his3; pRS315-sec21-3; YEp24-MST27 | This study |
| HHY216 | MATα; trp1; leu2; suc2-Δ9; sec21::his3; pRS315-sec21-3; YEp24-PRM8 | This study |
| HHY217 | Matα; ade2-101oc; his3-Δ200; leu2-Δ1; lys2-801; trp1-Δ63; ura3-52; YGL051w::GAL-myc3-YGL051w(HIS3) | This study |
| HHY218 | Matα; ade2-101oc; his3-Δ200; leu2-Δ1; lys2-801; trp1-Δ63; ura3-52; YGL053w::GAL-myc3-YGL053w(HIS3) | This study |
| HHY251 | MATα; trp1; leu2; suc2-Δ9; sec21::his3; pRS315-sec21-3; YEp24-MST27-AAXX | This study |
| Y10422 | Matα; leu2Δ0; lys2Δ0; ura3Δ0; YAR031w::kanMX4 | Euroscarf |
| Y14419 | Matα; his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0; YGL051w::kanMX4 | Euroscarf |
| Y14420 | Matα; his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0; YGL053w::kanMX4 | Euroscarf |
| Y34419 | Mat a/α; his3Δ1/his3Δ1; leu2-Δ1/leu2-Δ1; lys2Δ0/LYS2; MET15/met15Δ0; ura3Δ0/ura3Δ0; YGL051w::kanMX4/YGL051w::kanMX4 | Euroscarf |
| Y34420 | Mat a/α; his3Δ1/his3Δ1; leu2Δ0/leu2Δ0; lys2Δ0/LYS2; MET15/met15Δ0; ura3Δ0/ura3Δ0; YGL053w::kanMX4/YGL053w::kanMX4 | Euroscarf |
| Y30422 | Mat a/α; HIS3/his3Δ1; leu2Δ0/leu2Δ0; lys2Δ0/LYS2; MET15/met15Δ0; ura3Δ0/ura3Δ0; YAR031w::kanMX4/YAR031w::kanMX4 | Euroscarf |
| YAS254 | Mat α; ade2-101oc; his3-Δ200; leu2-Δ1; lys2-801; trp1-Δ63; ura3-52; YGL053w::GAL-myc3-YGL053w(HIS3); pPESC-MST27 | This study |
| YAS276 | Mat a/α; ade2-101/ade2-101; his3-Δ200/his3-Δ200; leu2-Δ1/leu2-Δ1; lys2-801/LYS; trp1-Δ63/trp1-Δ63; ura3-52/ura3Δ2; YGL089c::His12-YGL089c(URA3)/YGL089; YGL051w::HIS3/YGL051w::HIS3; YAR033w::HIS3/YAR033w::HIS3 | This study |
| YAS277 | Mat a/α; his3Δ1/his3Δ1; leu2Δ0/leu2Δ0; lys2Δ0/LYS2; MET15/met15Δ0; ura3Δ0/ura3Δ0; YGL053w::kanMX4/YGL053w::kanMX4; YAR031w::kanMX4/YAR031w::kanMX4 | This study |
| YAS286 | MAT α; ade2-101; his3-Δ200; leu2-Δ1, lys2-801; trp1-Δ63; ura3-52; pPESC-MST27 | This study |
| YAS308 | MAT α; ade2-101; his3-Δ200; leu2-Δ1, lys2-801; trp1-Δ63; ura3-52; YOL044w::GAL-myc3-YOL044w; pPESC-MST27 | This study |
| YAS314 | Mat α; ade2-101oc; his3-Δ200; leu2-Δ1; lys2-801; trp1-Δ63; ura3-52; YGL051w::GAL-myc3-YGL051w(HIS3); pSTM22 (URA3) | This study |
| YAS315 | Matα; ade2-101oc; his3-Δ200; leu2-Δ1; lys2-801; trp1-Δ63; ura3-52; YGL053w::GAL-myc3-YGL053w(HIS3); pSTM22 (URA3) | This study |
| YAS316 | MATα; ade2-101; his3-Δ200; leu2-Δ1, lys2-801; trp1-Δ63; ura3-52; pSTM22 (URA3) | This study |
| YPH499 | MAT a; ade2-101; his3-Δ200; leu2-Δ1, lys2-801; trp1-Δ63; ura3-52 | Phil Hieter |
| YPH500 | MAT α; ade2-101; his3-Δ200; leu2-Δ1, lys2-801; trp1-Δ63; ura3-52 | Phil Hieter |